Table 2.
Horizontal transmission of BcPV2 from QT5-19 to isolates of B. cinerea (B05.10, 08168, RoseBc-3, XN-1) and effect of BcPV2 infection on mycelial growth, conidial production, sclerotial production, and pathogenicity of the recipients.
| Isolate | BcPV2 x | Mycelial Growth Rate (cm/day) y | Conidial Production y | No. Sclerotia per Dish y | Lesion Diameter (cm) z |
|---|---|---|---|---|---|
| QT5-19 | + | 1.2 | - | 0 | 0.2 |
| B05.10 | - | 1.5 | + | 36 | 3.1 |
| B05.10T | + | 1.5 | - | 0 ** | 2.2 * |
| 08168 | - | 1.5 | + | 38 | 3.8 |
| 08168T | + | 1.5 | - | 0 ** | 2.3 * |
| XN-1 | - | 1.5 | + | 37 | 4.0 |
| XN-1T | + | 1.5 | - | 0 ** | 2.9 * |
| RoseBc-3 | - | 1.4 | + | 25 | 2.3 |
| RoseBc-3T | - | 1.4 | + | 22 | 2.2 |
x BcPV2 was detected by dsRNA profiling and RT-PCR using the specific primer pairs RdRp-f1/RdRp-r1 (dsRNA 1) and CP-f1/CP-r1 (dsRNA-2). “+”, the presence of BcPV2; “-”, the absence of BcPV2. y Mycelial growth rate, conidial production, and sclerotial production were determined on PDA at 20 °C. Each value is an average of 5 replicates. “+” with conidia, “-” without conidia. “**” indicates significant difference at p < 0.01 between each recipient and its transfected derivative according to Student’s t test. z Pathogenicity was determined on detached tobacco leaves (20 °C, 72 h). Each value of lesion diameter is an average of seven replicates. “*” indicates significant difference at p < 0.05 between each recipient and its transfected derivative isolate according to Student’s t test.