Figure 4.
Effect of PSC-CM on pancreatic cancer cell proliferation and migration. (A) Cancer cell proliferation: BxPC-3 and MIA PaCa-2 cells were incubated with SFM or PSC-CMs for 24 h, and DNA synthesis was determined by [3H]-thymidine incorporation assay. Data are mean ± SEM of triplicate determinations. (B) Cancer cell migration: BxPC-3 and MIA PaCa-2 cells were cultured to confluence, and scratch wounds were established. Images of the wound area were taken immediately after the scratches and 24 h after incubation with SFM or PSC-CMs. The wound area was measured using FIJI software. Data are mean ± SEM of eight scratches for each PSC-CM. # p < 0.1, * p < 0.05, ** p < 0.01 comparing control (SFM) and PSC-CM. PSC, pancreatic stellate cell; hPSC, human primary PDAC-derived PSC culture; HPaSteC, PSCs from normal human pancreas; i-hPSC, immortalized human PSCs; i-mPSC C2 and C3, immortalized mouse PSCs clone 2 and 3; PSC-CM, PSC-conditioned medium; SFM, serum-free DMEM.