Table 3.
Results from RT-qPCR analysis.
| miRNA | Tissue 1 | ΔΔCT 2 | Enrichment 3 |
|---|---|---|---|
| ssa-miR-9a-5p 4 | B | −11.13 | 2241 |
| ssa-miR-9b-3p | B | −10.21 | 1184 |
| ssa-miR-96-5p | B | −5.18 | 36 |
| ssa-miR-129-5p | B | −3.56 | 12 |
| ssa-miR-132-5p | B | −7.46 | 176 |
| ssa-miR-135c-5p | B | −7.05 | 133 |
| ssa-miR-153a-3p | B | −10.08 | 1082 |
| ssa-miR-212ab-3p | B | −7.29 | 156 |
| ssa-miR-219a-3p | B | −7.66 | 202 |
| ssa-miR-723-5p | B | −6.83 | 114 |
| ssa-miR-734a-3p | B | −4.68 | 26 |
| ssa-miR-122-5p4 | L | −12.3 | 5043 |
| ssa-miR-8163-3p | L | −8.6 | 388 |
| ssa-miR-192a-5p 4 | L | −7.96 | 249 |
| ssa-miR-499a-5p 4 | H | −9.7 | 832 |
| ssa-miR-736-3p | H | −14.7 | 26616 |
| ssa-miR-192a-5p 4 | I | −11.9 | 3822 |
| ssa-miR-459-5p | I | −12.9 | 7643 |
| ssa-miR-194b-5p | I | −10.8 | 1783 |
| ssa-miR-140-3p | G | −3.7 | 13 |
1 Tissue where the particular mature miRNA is highly expressed (B = brain, L = liver, H = heart, I = intestine, G = gills, M = muscle). 2 Logfold difference in highly expressed tissue relative to mean of all other. 3 Times increase in the highly enriched tissue.4 These four miRNAs are also among those shown in Figure 4. All differences were significant at adjusted p-values ≤ 0.05.