Figure 4.

Reduced expression of Wt1, Srpk1, and Srsf1 and altered Vegf splicing in tumor endothelial cells of Tie2-Cre^ERT2; Wt1^Lox/Lox mice treated with Tamoxifen compared to vehicle-treated controls. (a) Expression levels of Wt1, Srpk1, and Srsf1 were determined by quantitative RT-PCR on isolated endothelial cells of tumors from Tie2-Cre^ERT2; Wt1^Lox/Lox mice treated with Tamoxifen compared to vehicle-treated controls (n = 6 each). Expression of each gene was normalized to the respective arithmetic means of Gapdh, Actnb, and Rplp0 expression. (b) Expression of the different Vegf isoforms (Vegf 188: 293 bp; Vegf 164: 221 bp; Vegf 120: 89 bp) was determined on isolated endothelial cells (n = 3 each) as described [18]. (c) Quantification of relative band intensities revealed relative higher Vegf120 isoform levels, while Vegf164 and Vegf188 were not significantly affected by the knockout of Wt1. Data are expressed as means ± S.E.M. * p < 0.05, ** p < 0.01.