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Addition-time effect of 20 mM NH4Cl on influenza virus infection. (A) NH4Cl was added to the medium immediately after the attachment step, followed by incubation for different lengths of time. (B) After virus attachment, cells were incubated for various periods of time before the addition of NH4Cl. Six hours after viral infection, the synthesis of viral antigens was assessed by indirect immunofluorescence.
