Figure 5.

Comparative study between three protein quantification techniques, RP-HPLC, HPLC-ELSD and BCA assay. All three liposomal formulations were made using microfluidics. The DSPC:Chol and DSPC:Chol:PS formulations were made at a 3:1 FRR and 15 mL/min TFR (4 mg/mL initial lipid and 0.25 mg/mL initial ovalbumin concentration). The DSPC:Chol:DOTAP formulation was produced at a 1:1 FRR, and the ovalbumin was adsorbed onto the surface by passing pre-made DSPC:Chol:DOTAP formulation through the microfluidics NanoAssemblr. All results were measured three times, with the average encapsulation and ovalbumin loading calculated.