Table 1.
Half maximal inhibitory concentration (IC50) [μg/mL] of the extracts of E. menziesii against nervous system cancer cell lines.
| Cell Line | Hx | Chl | MeOH | ||||||
|---|---|---|---|---|---|---|---|---|---|
| 4 h | 24 h | 48 h | 4 h | 24 h | 48 h | 4 h | 24 h | 48 h | |
| BC | >1000 a | >1000 c | >1000 g | >1000 a | >1000 e | >1000 i | >1000 a | >1000 b | >1000 c |
| C6 | 9.51 ± 1.45 b | 9.59 ± 1.34 d | 8.59 ± 0.93 h | 9.82 ± 0.83 b | 8.86 ± 1.23 f | 7.39 ± 1.43 j | >1000 a | >1000 b | >1000 c |
| MIO-M1 | >1000 a | 88.48 ± 1.65 e | 10.08 ± 1.98 i | 90.11 ± 1.23 c | 86.32 ± 1.39 g | 9.41 ± 1.93 k | >1000 a | >1000 b | >1000 c |
| N1-115 | 10.94 ± 1.93 b | >1000 c | >1000 g | >1000 a | >1000 e | >1000 i | >1000 a | >1000 b | 10.23 ± 1.23 d |
| U737 | >1000 a | 891.96 ± 1.23 f | 906.73 ± 1.73 j | 105.71 ± 1.83 d | 108.85 ± 1.93 h | 95.76 ± 1.35 l | >1000 a | >1000 b | >1000 c |
BC: Bergmann glia primary culture cells; C6: Rattus norvegicus glioma cell line; MIO-M1: immortalized human Müller cell line; N1-115: Mus musculus neuroblastoma cell line; U737: human glioblastoma cell line; Hx: Hexanic extract; Chl: chloroformic extract; MeOH: methanolic extract. IC50 was determined as described in the Materials and Methods section. Two-way ANOVA and Tukey’s tests with multiple comparisons were performed for each extract tested, considering α value of 0.05. a–l nomenclature: the same superscript lowercase letters mean there are no significant differences among the values of each treatment; conversely, distinct letters indicate significant differences among treatments.