Table 2.
Panel of significantly altered metabolites 7 days after dosing with palbociclib (n = 4), fulvestrant (n = 4), or a combination dose of both palbociclib and fulvestrant (n = 4) when comparing dosed groups to control. Values are log transformed fold changes after quantification by multiple reaction monitoring, unpaired two-tailed t-test.
| Metabolite Name | Palbociclib | Fulvestrant | Combination | |||
|---|---|---|---|---|---|---|
| Fold Change | p-Value | Fold Change | p-Value | Fold Change | p-Value | |
| Sedoheptulose-7-phosphate | 3.5 | 0.0076 | 13.2 | <0.0001 | 6.4 | 0.0012 |
| NADP | N.S | N.S | −2.6 | 0.0168 | −3.7 | 0.0030 |
| Citrate/Isocitrate | 9.3 | <0.0001 | 5.3 | <0.0001 | 3.8 | 0.0017 |
| Succinate | −1.9 | 0.0047 | −1.7 | <0.0001 | −1.8 | 0.0356 |
| Malate | 2.4 | 0.0130 | N.S | N.S | 1.7 | 0.0323 |
| Fructose-1-phosphate | 5.8 | 0.0036 | 19.5 | 0.0002 | 11.2 | 0.0006 |
| N-acetylaspartylglutamate | N.S | N.S | −3.4 | 0.0030 | −2.6 | 0.0051 |
| Aspartate | N.S | N.S | −2.6 | 0.0021 | −2.3 | 0.0041 |
| Serine | N.S | N.S | −2.7 | 0.0370 | −3.2 | 0.0245 |
| Proline | N.S | N.S | N.S | N.S | −2.5 | 0.0014 |
| Asparagine | N.S | N.S | −5.5 | 0.0021 | −8.4 | 0.0003 |
| Tryptophan | N.S | N.S | N.S | N.S | −2.9 | 0.0134 |
| Tyrosine | −10.0 | 0.0138 | −1008.1 | <0.0001 | −696.5 | <0.0001 |
| Phenylalanine | N.S | N.S | 4.2 | 0.0007 | 3.5 | 0.0008 |
| Valine | 1.8 | 0.0050 | 1.5 | 0.0003 | 1.7 | <0.0001 |
| Arginine | N.S | N.S | N.S | N.S | −3.5 | 0.0174 |
| Guanosine | −1.3 | 0.0496 | N.S | N.S | −4.5 | <0.0001 |
| Inosine monophosphate | −33.3 | <0.0001 | −37.3 | 0.0032 | −29.2 | 0.0021 |
| Adenosine monophosphate | −8.5 | 0.0008 | −11.1 | 0.0017 | −10.9 | 0.0019 |