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. 2019 Jan 14;10(1):82–96. doi: 10.1080/21505594.2018.1559673

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© 2019 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 1. — LPS profile of O2 serotype-converted mutants in S. Typhimurium.

(a) The LPS profiles of the wild-type S. Paratyphi A and S. Typhimurium and mutants S1049 (Δabe::prt-tyv_A1), S1071 [Δ(abe-wzx_B1)::(prt-wzx_A1)], S1072 (Δabe::prt-tyv_A1 ΔwbaV_B1:: wbaV_A1) and S1074 [Δ(abe-wbaV_B1)::(prt-wbaV_A1)] were visualized by silver staining. (b and c) Western blot analysis confirmed the O-serotype conversion from O4 to O2. Salmonella O4 and O2 single-factor rabbit antisera were used as primary antibodies in the Western blot assay. Samples derived from the same experiment and gel/blots were processed in parallel.