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. 2016 Apr 17;139(5):1378–1393. doi: 10.1093/brain/aww079

Figure 3.

Figure 3

Severely reduced to absent SYNE1 staining in muscle tissue of SYNE1 patients . Immunohistological findings in control tissue ( A , E and I ), as well as in the quadriceps muscle biopsies of three different SYNE1 patients from three different countries: Patient 13-1, Belgian ( B , F and J ); Patient 10-1, German ( C , G and K ); and Patient 6-1, Italian ( D , H and L ). All three patients show a severely reduced to absent staining of the nuclear envelope after immunolabelling of nesprin-1 ( B – D ), whereas staining was normal in the control ( A ) (peroxidase-antiperoxidase technique). To control for an unspecific lack of staining in the SYNE1 patients, further control stainings were performed. Immunolabelling of emerin and lamin A/C at the inner nuclear membrane was normal in all three patients as well as the control ( E – H and I – L , respectively) (avidin-biotin complex technique).