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. Author manuscript; available in PMC: 2019 Feb 6.
Published in final edited form as: Neuropharmacology. 2018 Nov 2;145(Pt A):123–130. doi: 10.1016/j.neuropharm.2018.10.041

Fig. 2.

Fig. 2.

Effect of AICAR- or vehicle-treated Conditioned Medium on aNPCs. (A) MTT analysis of the reconstituted fractions from vehicle (V3, V4) or AICAR (A9, A11) conditioned medium (10 μg/μl, respectively) on aNPCs for 24 h. Fetal Bovine Serum (FBS), was used as a positive control. Same volume of distilled water was used as a vehicle (Veh). The FBS (10%) treated samples were significantly different from all other groups (*P < 0.05). (B–D) Real-time PCR analysis of neuronal cell differentiation makers (Tuj1, DCX, NeuN), with administration of reconstituted fractions from vehicle or AICAR treated culture medium (10 μg/μl, respectively) or not treated (NT), on aNPCs for 24 h (B) Tuj1 levels in A11 treated samples were higher than in all other groups. (C) DCX was elevated in the cells treated with fraction A11 as compared to V3, V4, A9 and NT. (D) NeuN levels were enhanced by FBS (0.5%) as compared to all other conditions. Data is expressed as relative target gene expression levels compared with HSP90 expression and presented as the mean of at least three independent experiments, each conducted in triplicate (*P < 0.05).