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. Author manuscript; available in PMC: 2019 Dec 13.
Published in final edited form as: Cell. 2018 Dec 13;175(7):1917–1930.e13. doi: 10.1016/j.cell.2018.08.044

FIGURE 1. An affinity tag-purification mass spectrometry approach for the study of EBOV virus protein-protein interactions.

FIGURE 1.

(A) A schematic representation of the AP-MS approach for identifying EBOV-human protein-protein interactions in HEK293T and Huh7 cells.

(B) A schematic representation of the EBOV genome and the principal functions of each encoded viral protein. The secreted glycoproteins (sGP and ssGP) are not represented and were not examined in this study.

(C) Silver stain of the eluted fraction following Strep affinity purification of the indicated bait proteins from HEK293T cells. Red arrows denote the bait protein in each lane.

(D) Bar graph summarizing the number of human proteins identified as interacting with each of the indicated EBOV baits. See also Supplemental Table S1.

(E) Heat map of KEGG terms significantly enriched (p-value < 0.05) among the human interacting proteins of the indicated EBOV baits. Darker shading indicates a more significant enrichment of the given KEGG term. There were no significantly enriched KEGG terms among the human proteins interacting with VP40 or NP. KEGG terms are clustered on the vertical axis by correlation between enrichment profiles across the depicted baits.

(F) Heat map of protein domain types significantly enriched (p-value < 0.05) among the human interacting proteins of the indicated EBOV baits. Darker shading indicates a more significant enrichment of the given domain type. There were no significantly enriched domain types among the human proteins interacting with VP40 or NP. KEGG terms are clustered on the vertical axis by correlation between enrichment profiles across the depicted baits.

(G) Venn diagram demonstrating the overlap in identified PPIs between this study and a previously published study by Garcia-Dorival et al. that focused on VP24 interactions in HEK293T cells. Nine PPIs were conserved among both studies as listed on the right.