Skip to main content
. Author manuscript; available in PMC: 2019 Mar 3.
Published in final edited form as: Oncogene. 2018 Sep 3;38(6):838–851. doi: 10.1038/s41388-018-0462-2

Figure 5: Ate1 reduction increases spontaneous and stress-induced mutagenesis in PC-3 cells.

Figure 5:

(A) Representative flow diagrams of PC-3 cells carrying CherryOFF-GFP reporter and pLKO.1-puro shRNA targeting Ate1 (shRNA #2) or NS control are shown. Cells were either treated with 3mM ENU for 24 hours, or irradiated with 10Gy of gamma radiation. 72 hours after the initiation of treatments, cells were analyzed by FACS for the acquired mutations that activate the mCherryFP fluorescence (indicated in the window of “Positive Cells”). (B) Quantification of A from three independent repeats shows the changes of mutation rates with the knockdown of Ate1. Error bars represent SEM. Statistical significances of the two-group comparison were assessed with the Student’s t-test: * = p<0.05, ** = p<0.01, *** = p<0.001.