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. Author manuscript; available in PMC: 2020 Feb 12.
Published in final edited form as: Biochemistry. 2018 Dec 27;58(6):665–678. doi: 10.1021/acs.biochem.8b00716

Table 1:

l-Cysteine adenylation assay – kinetic parameters

PchF-FLa PchF-FL PchF-AMT G667I-PchF-AMT
kcat (min−1) 415 2.6 ± 0.2 3.2 ± 0.2 3.0 ± 0.3
KM (l-Cys μM) 537 25 ± 2 41 ± 8 54 ± 6
kcat/KM(M−1s−) 13300 1800 ± 200 1300 ± 300 900 ± 100
a

Quadri, Keating, Patel, Walsh. Biochemistry 1999, 38, 14941–14954. Quadri et al used an exchange assay measuring the reverse reaction that incorporates 32P-pyrophosphate into ATP.