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Methylated product formation assay varying HPToxTred-CO2Et – kinetic parameters
| PchF-FL | PchF-AMT | G667I-PchF-AMT | |
|---|---|---|---|
| kcat (min−1) | 1.96 ± 0.04 | 2.9 ± 0.1 | Not Detected |
| KM (AdoMet μM) | 3.6 ± 0.2 | 7.5 ± 0.2 | Not Detected |
| kcat/KM (M−1s−1) | 9100 ± 500 | 6500 ± 300 | Not Detected |
