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IFN subtypes converge in their anti-HIV activities at 1,000 pg/ml, with the exception of IFN-α1. PBMCs were pretreated with the IFN-α subtypes and infected with HIV YU-2 overnight. The next day, the cultures were washed and IFN-αs added back. Vres was assessed for all subtypes (n = 3 donors, in duplicates). Significance testing was done using RM one-way ANOVAs, comparing all IFN-α treatment groups to IFN-α2.
