. Author manuscript; available in PMC: 2019 Feb 16.

Published in final edited form as: Mitochondrion. 2017 Jun 24;37:8–16. doi: 10.1016/j.mito.2017.06.003

Table 1.

Specifications and coefficients used to drive the accuracy of membrane potential measurements by fluorescence microscopy.

Microscope system		TMRM characteristics
Illumination	120 W-mercury lamp	Absorption/emission	549/574 nm
Filter cube Objective lens^a	TRITC Oil immersion 60 × Plan-Apochromat NA = 1.4	Quantum yield Extinction coefficient Absorption cross section	0.34 1.1 × 10⁵ M⁻¹ cm⁻¹ (kenmoku et al., 2007) 3.824 × 10⁻¹⁹ cm²
	Field number: 22 mm FOI = 0.001 cm²	Concentration (nonquench)	1–30 nM

Mitochondrial matrix volume: 0.04–0.08 µm ⁻³ (Kaasik et al., 2007).