Table 1. CD8⁺ T-cell epitope selection for NY-ESO-1, MAGE-A3 and MAGE-A10 .

HLA class I	IEDB	Position	Length	Method	ic50 score	Percentile rank	E-total (kJ/mol)	Published
NY-ESO-1
A*02:01	SLLMWITQC	157-165	9	ann	32.98	0.1	-540.1	Yes 34
A*30:01	AARASGPGG	50-58	9	ann	21.2	0.1	-559.95	No
A*32:01	RLLEFYLAMP	86-98	10	ann/smm	36.08	0.5	-418.27	No
B*07:02	TPMEAELARR	98-109	10	ann	11.90	0.1	-490.14	No
B*40:02	KEFTVSGNI	124-132	9	ann	34.4	0.2	-552.45	No
B*40:02	RLLEFYLAMP	85-98	10	ann	17.0	0.2	-539.32	YES 35
B*55:01	MPFATPMEA	94-102	9	ann	23.70	0.2	-419.10	No
MAGE-A3
A*02:01	KVAELVHFL	112-120	9	ann	0.1	45.93	-374.92	Yes 36
A*02:01	FLWGPRALV	271-279	9	ann	0.1	12.02	-591.74	Yes 37
A*24:02	IMPKAGLLI	195-203	9	ann	0.1	-	-529.78	Yes 38
A*01:01	ELMEVDPIGHL	165-174	9	ann	0.1	34.74	-648.0	No
A*03:01	RKVAELVHFLL	111-122	10	ann	0.1	46.43	-457.78	No
A*02:06	GSVVGNWQYF	137-148	10	ann	0.1	45.46	-486.05	No
MAGE-A10
A*01:01	KLLTQDWVQ	269-280	9	ann	44.11	0.1	-489.12	No
A*01:01	NGSDPRSFPL	319-330	10	ann	54.21	0.1	-552.37	No
A*02:01	SLLKFLAKV	310-318	9	ann	8.90	0.4	-663.25	Yes 39
A*02:01	GLYDGMEHL	254-262	9	ann	9.09	0.8	-432.16	Yes 39
A*03:01	LLFKYQMKE	145-153	9	ann	47.96	0.1	-664.36	No
A*03:01	RAHAEIRKMS	301-313	10	ann	54.20	0.1	-699.67	No
A*11:01	MLSDVQSMPK	214-223	10	ann	12.72	0.2	-533.53	No
A*31:01	KFLAKVNGSD	313-324	10	ann	39.87	0.1	-502.90	No
A*31:01	RSFPLWYEEA	324-335	10	ann	29.13	0.1	-551.39	No
A*31:01	VIWEALNMMG	245-256	10	ann	53.0	0.2	-438.42	No

* Selected as the highest peptide binding affinity.

The selected sequences are shown in bold.