Skip to main content
. 2017 Aug 10;170(4):800–814.e18. doi: 10.1016/j.cell.2017.07.031

Figure 2.

Figure 2

Inducible iChr-Notch-Mosaic ES Cells and Mice

(A) iChr-Notch-Mosaic DNA construct inserted in the Rosa26 locus. Below the genetic distance (kb) between different LoxP sites and the relative recombination ratios obtained after Cre-expressing plasmid transfection.

(B) Representative picture of iChr-Notch-Mosaic ES cells expressing the different fluorescent proteins.

(C) Confocal micrographs of 20-micron thick cryosections immunostained for the indicated markers. The border between the marginal and ventricular zones is indicated by a yellow line (see also Figure S3).

(D) Dot plot of individual clones. Error bars indicate SEM.

(E) Ratios of the recombined cells observed in ES cells and after differentiation to ECs. Below is shown a representative picture of an embryoid body (EB)-derived endothelial monolayer.