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. 2019 Feb 1;146(3):dev171520. doi: 10.1242/dev.171520

Fig. 2.

Fig. 2.

Compartmentalization of redox state within the Drosophila larval midgut is sensitive to colonization with L. plantarum. (A) Detection of the ubiquitous expression of the redox-sensitive roGFP from the genotype tub-GAL4 UAS-cyto-roGFP2-Orp1 in the third instar larval midgut, in which reduced roGFP is excited at 405 nm (trend towards yellow) and oxidized roGFP is excited at 488 nm (trend towards blue). Note strong detection of oxidized (488 nm) roGFP surrounding AMP islets in conventionally raised, but not in germ-free larvae. Note reduced state of ECs in germ-free larvae. Colonization of germ-free larvae with L. plantarum (LP), but not with B. cereus (BC) induces the emergence of compartmentalization of redox states between the ECs and AMPs. (B) Magnified views of boxed areas of midgut regions outlined in A. Scale bars: 25 μm.