Figure 1. Validation of LepR reactivation in 10-week-old mice.

(A) Body weight of male mice (main effect of Genotype [F_{(2, 23)}=153.7, p<0.0001], main effect of Time [F_{(24, 552)}=22.37, p<0.0001] and interaction [F_{(48, 552)}=105.5, p<0.0001]; n = 6–12) before, during and after tamoxifen treatment at 10 weeks of age. (B) Food intake of male mice (main effect of Genotype [F_{(2, 23)}=14.13, p<0.0001], main effect of Time [F_{(23, 529)}=29.30, p<0.0001] and interaction [F_{(46, 529)}=18.58, p<0.0001]; n = 5–12). (C) Body weight of female mice (main effect of Genotype [F_{(2, 19)}=154.1, p<0.0001], main effect of Time [F_{(24, 456)}=23.75, p<0.0001] and interaction [F_{(48, 456)}=114.5, p<0.0001]; n = 2–16). (D) Food intake of female mice (main effect of Genotype [F_{(2, 15)}=3.779, p=0.0469], main effect of Time [F_{(23, 345)}=8.386, p<0.0001] and interaction [F_{(46, 345)}=5.435, p<0.0001]; n = 1–13). ### p<0.0001 Ubi-LepRNull vs LepRNull mice. ***p<0.0001 Ubi-LepRNull vs Ubi mice. (E–J) Brightfield photomicrographs of pSTAT3 immunoreactive neurons in Ubi (E, H), LepRNull (F, I) and Ubi-LepRNull mice (G, J) after an acute leptin injection (5 µg/g b.w.). 3 v, third ventricle. Scale Bar = 200 µm. (K) Number of pSTAT3 cells after an i.p. leptin injection (n = 5–6). ***p<0.001. (L) Acute changes in food intake (left panel) (F_{(2, 28)}=5.927, *p=0.0071, n = 8–13) and body weight (right panel) (F_{(2, 33)}=4.524, *p=0.0184, n = 8–18) after an i.p. leptin injection (2.5 µg/g b.w.) in comparison to PBS injection (Figure 1—source data 1).