Figure 2.

CuB reduces invasive behavior and induces apoptosis of GR NSCLC cells. (A) Invasion assay was carried out using modified 24-well microchemotaxis chambers. H1975 cells were pretreated with CuB for 30 min. (B) Confluent H1975 cells were scratched and then treated with CuB in a basic medium for 24 h. (C) Statistical results of Figure 2A,B. Data are shown as the mean ± SD of three independent experiments.* P < 0.05; ** P < 0.01 vs. 0 μM. (D) H1975 and H820 cells were treated with increasing concentrations of CuB for 24 h. Western blot was performed using antibodies indicated. GAPDH was used as the loading control.