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. 2018 Dec 7;68(3):587–597. doi: 10.2337/db18-0955

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© 2018 by the American Diabetes Association.

Readers may use this article as long as the work is properly cited, the use is educational and not for profit, and the work is not altered. More information is available at http://www.diabetesjournals.org/content/license.

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Higher basal SMAD2P in Om ASCs negatively correlated with adipogenesis. A: Phosphorylated (PSMAD2) and total (TSMAD2) SMAD2, SMA, and HSP90 (a loading control) levels were measured in paired Om (O) and Abdsc (S) ASCs derived from 10 different donors at confluence before differentiation (top panels). ASCs derived from the same donors were differentiated, and markers of adipocytes, ATGL and ADPN, and HSP90 protein levels were measured on day 14 (bottom panels). B: Quantification of SMAD2P and differentiation (Diff.) degree as determined by ATGL (C) and ADPN (D) protein levels relative to HSP90. E: Negative correlations between basal SMAD2P and differentiation degree (ATGL). F: Quantification of SMA protein relative to HSP90 before differentiation. *P < 0.05, **P < 0.01 (paired t tests); n = 10. AU, arbitrary units.