Key resources table.
| Reagent type or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Species (Drosophila melanogaster) |
UAS-FLP | BDSC | #4539 | FLP enzyme under UAS control |
| Species (Drosophila melanogaster) |
R9D11-Gal4 | BDSC | #40731 | Young INP Gal4 driver |
| Species (Drosophila melanogaster) |
R37G12-lexA | BDSC | #52765 | PF-R lexA driver |
| Species (Drosophila melanogaster) |
R60D05-lexA | BDSC | #52867 | E-PG lexA driver |
| Species (Drosophila melanogaster) |
R12D09-lexA | BDSC | #54419 | P-EN lexA driver |
| Species (Drosophila melanogaster) |
R16D01-lexA | BDSC | #52503 | P-FN lexA driver |
| Species (Drosophila melanogaster) |
lexAop(FRT.stop)mCD8::GFP | BDSC | #57588 | FLP-out membrane bound GFP under lexAop control |
| Species (Drosophila melanogaster) |
ts.Tubulin-Gal80 (20) | BDSC | #7019 | temperature sensitive Gal80 |
| Species (Drosophila melanogaster) |
20xUAS-FLP.PEST | BDSC | #55807 | FLP enzyme under 20xUAS control |
| Species (Drosophila melanogaster) |
OK107-Gal4 | BDSC | #854 | Eyeless enhancer trap Gal4 for old INPs |
| Species (Drosophila melanogaster) |
UAS-mCherryRNAi | BDSC | #35787 | Control RNAi under UAS control |
| Species (Drosophila melanogaster) |
UAS-EyelessRNAi | BDSC | #32486 | Eyeless RNAi under UAS control |
| Species (Drosophila melanogaster) |
13xlexAop-myr::GFP | BDSC | #32210 | membrane bound GFP under 13xlexAop control |
| Species (Drosophila melanogaster) |
R16B06-Gal4 | BDSC | #45811 | old INP Gal4 driver |
| Species (Drosophila melanogaster) |
UAS-FLP, Act5c(FRT.CD2)Gal4; ; R12E09-Gal4, UAS-mCD8::GFP |
This work | INP immortalization driver expressing membrane bound GFP |
|
| Species (Drosophila melanogaster) |
lexAop(FRT.stop)HA::CD4.T2A.Brp.mCherry | BDSC | #56518 | FLP-out fluorensent labeling of Brp |
| Species (Drosophila melanogaster) |
ss00096-Gal4 | Rubin Lab (Janelia) | E-PG split Gal4 driver | |
| Species (Drosophila melanogaster) |
Empty-vector split Gal4 | Rubin Lab (Janelia) | Control split Gal4 driver | |
| Species (Drosophila melanogaster) |
UAS-ToyRNAi | BDSC | #33679 | Toy RNAi under UAS control |
| Species (Drosophila melanogaster) |
lexAop.tdTomato.myr, brp(FRT.stop)V5-2A-lexA-VP16 |
BDSC | #56142 | STaR FLP-out labeling of synaptic terminals |
| Species (Drosophila melanogaster) |
10xUAS-myr::HA | BDSC | #62145 | membrane bound HA under UAS control |
| Species (Drosophila melanogaster) |
R19G02-Gal4 | BDSC | #48860 | developmental E-PG Gal4 |
| Species (Drosophila melanogaster) |
UAS-Kir2.1 | Giraldo et al., 2018 | Inward rectifying K + channel under UAS control |
|
| Antibody, polyclonal | Chicken anti-GFP | Abcam (Eugene, OR) | 1:1000 | |
| Antibody, polyclonal | Rabbit anti-Toy | Desplan lab (NYU) | 1:1000 | |
| Antibody, polyclonal | Guinea-pig anti-Runt | Desplan lab (NYU) | 1:1000 | |
| Antibody, monoclonal | Mouse anti-nc82 | DSHB (Iowa City, IA) | 1:50 | |
| Antibody, polyclonal | Rabbit anti-V5 | Cell Signaling (Danvers MA) |
1:400 | |
| Antibody, polyclonal | Rabbit Anti HA | Columbia Biosciences (Frederick MD) |
1:400 | |
| Antibody, polyclonal | Secondary antibodies | Thermofisher (Eugene, OR) | 1:400 |