Key resources table.
| Reagent type (species) or recourse |
Designation | Source or reference |
Identifier | Additional information |
|---|---|---|---|---|
| Gene (Homo sapines) | FUS | NA | Gene ID: 2521 | |
| Strain, strain background (Mus musculus) | Mouse/B6N.Cg-Tg(Prnp-FUS-wt) line 101 | this study | murine prion promoter driven FUS cDNA (human wild type FUS) | C3H and C57BL/6 hybrid backcross to C57BL/6J for more than five generations |
| Strain, strain background (Mus musculus) | Mouse/B6N.Cg-Tg(Prnp-FUS-wt) line 136 | this study | murine prion promoter driven FUS cDNA (human wild type FUS) | C3H and C57BL/6 hybrid backcross to C57BL/6J for more than five generations |
| Strain, strain background (Mus musculus) | Mouse/B6N.Cg-Tg(Prnp-FUS-R514G) line 124 | this study | murine prion promoter driven FUS cDNA (human R514G FUS) | C3H and C57BL/6 hybrid backcross to C57BL/6J for more than five generations |
| Strain, strain background (Mus musculus) | Mouse/B6N.Cg-Tg(Prnp-FUS-R521C) line 135 | this study | murine prion promoter driven FUS cDNA (human R521C FUS) | C3H and C57BL/6 hybrid backcross to C57BL/6J for more than five generations |
| Strain, strain background (Mus musculus) | C57BL/6 mFUS-/- | Hicks et al., 2000 | C57BL/6J background | |
| Cell line (Mus musculus) | Neuro2A | ATCC | Cat# CCL-131, RRID:CVCL_0470 | Free of mycoplasma contamination. Species confirmed and authenticated by the Cytochrome oxidase 1 (CO1) barcode assay. |
| Transfected construct | pEBFP2-Nuc | Addgene | 14893, RRID:Addgene_14893 | |
| Transfected construct (Homo sapines) | BFP-wt-FUS | this study | ||
| Transfected construct (Homo sapines) | BFP-R514G-FUS | this study | ||
| Transfected construct (Homo sapines) | BFP-R521C-FUS | this study | ||
| Transfected construct (Homo sapines) | pDest-mCherry-EGFP-hLC3b | Addgene | 22418, RRID:Addgene_22418 | |
| Antibody | FUS | Santa Cruz | clone 4H11, sc-47711, RRID:AB_2105208 | 1:500 (WB), 1:100 (IF) |
| Antibody | human FUS, #14080, affinity purified | this study, Lopez-Erauskin et al., Neuron 2018 | peptide sequences for immunization, CKKKGSYSQQPSYGGQQ | 0.1 ug/ml (WB), 1 ug/ml (IF) |
| Antibody | mouse FUS, #14082, affinity purified | this study, Lopez-Erauskin et al., Neuron 2018 | peptide sequences for immunization, CKKKGGYGQQSGYGGQQ | 0.1 ug/ml (WB), 1 ug/ml (IF) |
| Antibody | HA | Bethyl Laboratories, Inc. | A190-238A, RRID:AB_2631898 | 1:5000 (WB), 1:1000 (IF) |
| Antibody | HA | Covance | mms-101P, RRID:AB_2314672 | 1:1000 (WB), 1:500 (IF) |
| Antibody | NeuN | Merck Millipore | MAB377, RRID:AB_2298772 | 1:1000 (IF) |
| Antibody | NeuN, Alexa-488 conjugate | Merck Millipore | MAB377X, RRID:AB_
2149209 |
1:1000 (IF) |
| Antibody | GFAP | Protien Tech | 16825–1-AP, RRID:AB_
2109646 |
1:1000 (IF) |
| Antibody | APC/CC1 | Merck Millipore | OP80, RRID:AB_2057371 |
1:1000 (IF) |
| Antibody | ChAT | Merck Millipore | AB144P, RID:AB_2079751 |
1:100 (IF) |
| Antibody | INPP4B | Cell Signaling Technology | #4039, RRID:AB_2126015 | 1:1000 (WB) |
| Antibody | KHC | Abcam | ab62104, RRID:AB_2249625 | 1:1000 (WB) |
| Antibody | HSP-90 | Enzo Life Sciences | ADI-SPA-846-D, RRID:AB_2039287 | 1:1000 (WB) |
| Antibody | Iba-I | Wako | 019–19741, RRID:AB_839504 | 1:1000 (IF) |
| Antibody | TDP-43 | ProteinTech | 10782–2-AP, RRID:AB_615042 | 1:1000 (IF) |
| Antibody | p62/sequestosome | Enzo Life Sciences | BML-PW9860, RRID:AB_2196009 | 1:500 (IF) |
| Antibody | BFP | abcam | ab32791, RRID:AB_873781 | 1:400 (IF) |
| Antibody | synaptophysin | Thermo Fisher Scientific | PA1-1043, RRID:AB_
2199026 |
1:50 (IF) |
| Antibody | Goat anti mouse alexa 350 | Thermo Fisher Scientific | A21049, RRID:AB_141456 | 1:100 (IF) |
| Antibody | Goat anti rabbit alexa 594 |
Thermo Fisher Scientific | A11037, RRID:AB_2534095 | 1:100 (IF) |
| Antibody | Donkey anti Rabbit FITC |
Jacksons Immunol | 711-095-152, RRID:AB_2315776 | 1:500 (IF) |
| Antibody | Donkey anti Rabbit Cy3 |
Jacksons Immunol | 711-165-152, RRID:AB_2307443 | 1:500 (IF) |
| Antibody | Donkey anti Rabbit Cy5 | Jacksons Immunol | 711-175-152, RRID:AB_2340607 | 1:500 (IF) |
| Antibody | Donkey anti Mouse FITC |
Jacksons Immunol | 715-095-151, RRID:AB_2335588 | 1:500 (IF) |
| Antibody | Donkey anti Mouse Cy3 | Jacksons Immunol | 715-165-151, RRID:AB_2315777 | 1:500 (IF) |
| Antibody | Donkey anti Mouse Cy5 | Jacksons Immunol | 715-175-151, RRID:AB_2619678 | 1:500 (IF) |
| Antibody | Donkey anti Goat FITC |
Jacksons Immunol | 705-095-147, RRID:AB_2340401 | 1:500 (IF) |
| Antibody | Donkey anti Goat Cy3 | Jacksons Immunol | 705-165-147, RRID:AB_2307351 | 1:500 (IF) |
| Antibody | Donkey anti Goat Cy5 |
Jacksons Immunol | 705-175-147, RRID:AB_2340415 | 1:500 (IF) |
| Recombinant DNA reagent | pcDNA5-FRT-TO-GFP-wt-FUS | Sun et al., 2015 | ||
| Recombinant DNA reagent | pcDNA5-FRT-TO-GFP-R514G-FUS | Sun et al., 2015 | ||
| Recombinant DNA reagent | pcDNA5-FRT-TO-GFP-R521C- FUS |
Sun et al., 2015 | ||
| Sequence-based reagent | qRT-PCR primers, mouse Chodl | IDT | forward, 5’-CCAGATGTTGCATAAAAGTAAAGGA-3’, reverse, 5’-TCCAGAACAATGCCAGTTCA-3’ | |
| Sequence-based reagent | qRT-PCR primers, mouse Ugt8a | IDT | forward, 5’- CGAAGGACGCGCTATGAAG-3’, reverse, 5’- CAAGGCCGATGCTAGTGTCT-3’ | |
| Sequence-based reagent | qRT-PCR primers, mouse Tesc | IDT | forward, 5’- TTGAAAAGGAGTCGGCTCGG-3’, reverse, 5’- CACCTGGTCCGGTTCCATC-3’ | |
| Sequence-based reagent | qRT-PCR primers, mouse Serpinb1a | IDT | forward, 5’- TGACTTTTGGCATGGGTATGTC-3’, reverse, 5’- GTCATGCAAAAGCCGAGGAG-3’ | |
| Sequence-based reagent | qRT-PCR primers, mouse Btbd17 | IDT | forward, 5’- GGGACTGTGCTGCTGTCTTT-3’, reverse, 5’- CTCACCACAGTACAAATACCTGATG-3’ | |
| Sequence-based reagent | qRT-PCR primers, mouse Gjc2 | IDT | forward, 5’- GCCTGGAGAAGGTCCCAC-3’, reverse, 5’- GTCAGCACAATGCGGAAGAC-3’ | |
| Sequence-based reagent | qRT-PCR primers, mouse Kcnip4 | IDT | forward, 5’- TTCATTGAAAGTTGCCAAAAA-3’, reverse, 5’- CTACAAGTGGGGGCTTCAAC-3’ | |
| Sequence-based reagent | qRT-PCR primers, mouse Nkain2 | IDT | forward, 5’- GGGCTTCATCTATGCCTGTT-3’, reverse, 5’- GATGTCTTCTGAGGCCCTTG-3’ | |
| Sequence-based reagent | qRT-PCR primers, mouse Park2 | IDT | forward, 5’- CAGACAAGGACACGTCGGTA-3’, reverse, 5’- GGGATCCCAGGAAGTCTTGT-3’ | |
| Sequence-based reagent | qRT-PCR primers, mouse Nrxn3 | IDT | forward, 5’- TTTCACCTGTGACTGCTCCA-3’, reverse, 5’- TTGCTGGCCAGGTATAGAGG-3’ | |
| Sequence-based reagent | qRT-PCR primers, mouse Pcdh9 | IDT | forward, 5’- GACAAGAGGACCGAAGCAGA-3’, reverse, 5’- GGTGTTGGTATGGACCCAAG-3’ | |
| Sequence-based reagent | qRT-PCR primers, mouse Grm7 | IDT | forward, 5’- GACTCGGGGTGTACCAGAGA-3’, reverse, 5’- TGGAGATTGTAAGCGTGGTG-3’ | |
| Sequence-based reagent | qRT-PCR primers, mouse Hs2st3 | IDT | forward, 5’- GGACGAGGACTGGACTGGTA-3’, reverse, 5’- GGGCTTCTTGAGTGACGAAA-3’ | |
| Sequence-based reagent | qRT-PCR primers, mouse Chat | IDT | forward, 5’- TCCGCTTCCGAGATGTTTCC-3’, reverse, 5’- AACATAGGGCCGGTTCCTTC-3’ | |
| Sequence-based reagent | qRT-PCR primers, mouse Nefl | IDT | forward, 5’- TGAGCCCTATTCCCAACTATTCC-3’, reverse, 5’- GGTTGACCTGATTGGGGAGA-3’ | |
| Sequence-based reagent | qRT-PCR primers, mouse Nefm | IDT | forward, 5’- CCATCCAGCAGTTGGAAAAT-3’, reverse, 5’- CGGTGATGCTTCCTGAAAAT-3’ | |
| Sequence-based reagent | qRT-PCR primers, mouse Mefh | IDT | forward, 5’- CAGCTGGACAGTGAGCTGAG-3’, reverse, 5’- CAAAGCCAATCCGACACTCT-3’ | |
| Sequence-based reagent | qRT-PCR primers, mouse Prph | IDT | forward, 5’- TGTGCCATTGTCAGGAGTCAG-3’, reverse, 5’- CTGTCTGGTGTTCCTCTCTGG-3’ | |
| Sequence-based reagent | qRT-PCR primers, mouse Chga | IDT | forward, 5’- GGTGCTGGACTTGGGATAGG-3’, reverse, 5’- CAGAGACAATGCCCCCACTC-3’ | |
| Sequence-based reagent | qRT-PCR primers, mouse Iift1 | IDT | forward, 5’- GCATCACCTTCCTCTGGCTAC-3’, reverse, 5’- GAATGGCCTGTTGTGCCAAT-3’ | |
| Sequence-based reagent | qRT-PCR primers, mouse Irf7 | IDT | forward, 5’- ACCCAAGGGGCCTTATTTGC-3’, reverse, 5’- TCTACACAGGCAGTCTGGGA-3’ |
|
| Sequence-based reagent | qRT-PCR primers, mouse Lcn2 | IDT | forward, 5’- AGCCACCATACCAAGGAGCA-3’, reverse, 5’- GGGGAGTGCTGGCCAAATA-3’ |
|
| Sequence-based reagent | qRT-PCR primers, mouse Iift3 | IDT | forward, 5’- TGAGGACAACCGGAAGTGTG-3’, reverse, 5’- TTTTCAGCACATTCTCCCCA-3’ | |
| Sequence-based reagent | qRT-PCR primers, mouse Gbp2 | IDT | forward, 5’- GACCAGAGTGGGGTAGACGA-3’, reverse, 5’- AAGGTTGGAAAGAAGCCCACA-3’ | |
| Sequence-based reagent | qRT-PCR primers, mouse Osal2 | IDT | forward, 5’- TCCTGACGACCTCGTTTTGG-3’, reverse, 5’- TCCTGACGACCTCGTTTTGG-3’ | |
| Sequence-based reagent | qRT-PCR primers, mouse Ctss | IDT | forward, 5’- ATCACTGCGGAATTGCTAGTT-3’, reverse, 5’- ACGACACACTTGGTTCCTCT-3’ | |
| Sequence-based reagent | qRT-PCR primers, mouse Laptm5 | IDT | forward, 5’- TCTCTGCCCCCTAAGACTCC-3’, reverse, 5’- CCTGGTGGGGATCACACTTC-3’ | |
| Sequence-based reagent | qRT-PCR primers, mouse Ctsc | IDT | forward, 5’- CTGCTTTCCCTACACAGCCA-3’, reverse, 5’- ACGGAGGCAATTCTCCCTTG-3’ | |
| Sequence-based reagent | qRT-PCR primers, mouse Slc11a1 | IDT | forward, 5’- CATCCAGCAAGCAAAGAGGC-3’, reverse, 5’- TCCAGAAAGCCAGTAGGGGA-3’ |
|
| Sequence-based reagent | qRT-PCR primers, mouse Ctsh | IDT | forward, 5’- AGACCAAGGGAGGAACTGGT-3’, reverse, 5’- GGTGGGCTTGTCGCTATTCA-3’ | |
| Sequence-based reagent | qRT-PCR primers, mouse Cd14 | IDT | forward, 5’- GAATTGGGCGAGAGAGGACT-3’, reverse, 5’- TCCTGACGACCTCCGCTAAAACTTGGAGGGTCG-3’ | |
| Sequence-based reagent | qRT-PCR primers, mouse Dusp5 | IDT | forward, 5’- ACTTCAGACCATCCCCAAGG-3’, reverse, 5’- TGAGGTGCAAGGACTAGGTG-3’ | |
| Sequence-based reagent | qRT-PCR primers, mouse Flnc | IDT | forward, 5’- AAAGAGCAATGGAAGACGGC-3’, reverse, 5’- CCACACATCACATGCTGCTT-3’ | |
| Sequence-based reagent | qRT-PCR primers, mouse Myc | IDT | forward, 5’- TCAGACACGGAGGAAAACGA-3’, reverse, 5’- GTTCCTCCTCTGACGTTCCA-3’ | |
| Sequence-based reagent | qRT-PCR primers, mouse Rac2 | IDT | forward, 5’- CTTCCTGCCTGTTTTGGGTC-3’, reverse, 5’- ACCTGAACTTGACCTCGGAG-3’ | |
| Commercial assay or kit | SuperScript First-Strand Synthesis System | Thermo Fisher Scientific | 1800051 | |
| Commercial assay or kit | iQSYBR Green Supermix | Bio-Rad | 1708880 | |
| Commercial assay or kit | Illumina TruSeq RNA Sample Prep Kit | Illumina | RS-122–2001 | |
| Chemical compound, drug | α-Bugarotoxin, Alexa Fluor 488 conjugate | Thermo Fisher Scientific | B13422 | |
| Chemical compound, drug | Fluoromyelin Red Fluorescent Myelin stain | Thermo Fisher Scientific | F34652 | |
| Software, algorithm | GraphPad Prism 7.0 | GraphPad Software | RRID:SCR_002798 | |
| Software, algorithm | ComBat | Leek et al., 2012 | RRID:SCR_010974 | |
| Software, algorithm | Kallisto-Sleuth | Pachter lab, https://pachterlab.github.io/kallisto/ and https://pachterlab.github.io/sleuth/about | Kallisto: RRID:SCR_016582
sleuth: RRID:SCR_016883 |
|
| Software, algorithm | Cytoscape | Smoot et al., 2011 | RRID:SCR_003032 | |
| Software, algorithm | ClueGO | Bindea et al., 2009 | RRID:SCR_005748 | |
| Software, algorithm | Bioquant Software | BIOQUANT Life Science | RRID:SCR_016423 |