Figure 10.

HLA-G–inducing factors in ascitic fluids.

Hospicells, treated for 6 hours with different cytokines, were tested for sHLA-G induction by RT-PCR and flow cytometry:

(A) IL-1β and TGF-β were only found as inducers of sHLA-G.

(B, C). IL-1β was found to be more potent than TGF-β at concentrations as low as 1 ng/ml. The inducer effect of IL-1β was dose dependent, measured by FACS and RT-PCR.

(D) Signaling pathway inhibitors (JAK1, STA3, NF-κB, and MPkk) were used on hospicells in culture using a medium containing bovine calf serum. Total RNA was extracted after 6 hours of treatment, and RT-PCR was carried out. HLA-G by IL-1β was found to be induced through the NF-κB pathway.