Table 2. The stability of the expression of seven candidate reference genes in six groups as analysed by geNorm, NormFinder, BestKeeper.

Group	Rank	geNorm		NormFinder		BestKeeper
		Gene	Stability	Gene	Stability	Gene	SD	CV
Abiotic Cotyledons	1	FtCACS	0.33	FtH3	0.058	FtSAND	0.33	1.32
	2	FtH3	0.33	FtCACS	0.080	FtCACS	0.31	1.47
	3	FtSAND	0.41	FtSAND	0.129	FtH3	0.33	1.64
	4	FtExpressed1	0.54	FtExpressed1	0.280	FtExpressed1	0.59	2.48
	5	FtEF-1α	0.73	FtEF-1α	0.683	FtEF-1α	1.04	5.70
	6	FtGAPDH	0.84	FtGAPDH	0.751	FtGAPDH	1.07	6.26
	7	FtActin	1.31	FtActin	1.678	FtActin	1.46	6.36
Abiotic Hypocotyls	1	FtH3	0.65	FtSAND	0.416	FtH3	0.51	2.47
	2	FtSAND	0.65	FtCACS	0.437	FtSAND	0.69	2.68
	3	FtCACS	0.72	FtH3	0.449	FtActin	0.79	3.38
	4	FtExpressed1	0.84	FtEF-1α	0.469	FtExpressed1	0.81	3.34
	5	FtEF-1α	0.91	FtExpressed1	0.475	FtCACS	0.86	3.75
	6	FtGAPDH	1.05	FtGAPDH	0.811	FtEF-1α	0.92	5.32
	7	FtActin	1.17	FtActin	0.895	FtGAPDH	1.35	7.59
Abiotic total	1	FtH3	0.55	FtCACS	0.304	FtH3	0.52	2.55
	2	FtSAND	0.55	FtSAND	0.327	FtCACS	0.61	2.70
	3	FtCACS	0.61	FtH3	0.349	FtSAND	0.63	2.46
	4	FtExpressed1	0.72	FtExpressed1	0.380	FtExpressed1	0.73	3.02
	5	FtEF-1α	0.89	FtEF-1α	0.694	FtEF-1α	1.02	5.71
	6	FtGAPDH	1.01	FtGAPDH	0.782	FtActin	1.17	5.07
	7	FtActin	1.30	FtActin	1.329	FtGAPDH	1.20	6.89
Al treatment	1	FtEF-1α	0.16	FtSAND	0.091	FtExpressed1	0.17	0.73
	2	FtExpressed1	0.16	FtExpressed1	0.100	FtEF-1α	0.25	1.55
	3	FtSAND	0.21	FtH3	0.105	FtH3	0.31	1.56
	4	FtGAPDH	0.23	FtEF-1α	0.106	FtGAPDH	0.34	1.88
	5	FtH3	0.27	FtGAPDH	0.156	FtCACS	0.35	1.57
	6	FtCACS	0.30	FtCACS	0.282	FtSAND	0.37	1.43
	7	FtActin	0.49	FtActin	0.652	FtActin	1.05	4.16
Immature seed stage	1	FtExpressed1	0.16	FtSAND	0.097	FtExpressed1	0.50	2.00
	2	FtCACS	0.16	FtGAPDH	0.097	FtCACS	0.57	2.43
	3	FtSAND	0.30	FtH3	0.167	FtSAND	0.82	3.03
	4	FtGAPDH	0.36	FtCACS	0.292	FtEF-1α	0.83	4.32
	5	FtH3	0.45	FtExpressed1	0.422	FtGAPDH	0.86	4.51
	6	FtEF-1α	0.62	FtEF-1α	0.675	FtH3	1.11	5.29
	7	FtActin	1.01	FtActin	1.354	FtActin	2.16	9.96
Total	1	FtH3	0.60	FtCACS	0.245	FtCACS	0.53	2.33
	2	FtCACS	0.60	FtSAND	0.304	FtH3	0.54	2.69
	3	FtSAND	0.66	FtH3	0.313	FtSAND	0.57	2.24
	4	FtExpressed1	0.71	FtExpressed1	0.377	FtExpressed1	0.68	2.85
	5	FtGAPDH	0.87	FtGAPDH	0.650	FtGAPDH	0.97	5.53
	6	FtEF-1α	0.97	FtEF-1α	0.780	FtEF-1α	1.03	5.91
	7	FtActin	1.33	FtActin	1.480	FtActin	1.36	5.70