Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| ene (E. coli) | AdoHcy nucleosidase | NA | NCBI: NC_000913.3 | |
| Gene (B. subtilis) | adenine deaminase | NA | NCBI: NC_000964.3 | |
| Gene (Homo sapiens) | β-actin | NA | GenBank: NM_001101.4 | |
| Gene (H. sapiens) | SETD3 | NA | NCBI: NM_032233.2 | |
| Recombinant DNA reagent |
pCOLD I (plasmid) | Takara Bio | 3361 | |
| Genetic reagent (E. coli) |
pCOLD I/β-actin | PMID: 20851184 | Kind gift from M. Tamura, Ehime University |
|
| Genetic reagent (E. coli) |
pCOLD I/AdoHcy nucleosidase | PMID: 30526847 | Overexpression of the recombinant E. coli AdoHcy nucleosidase in E. coli BL21 (DE3) |
|
| Genetic reagent (E. coli) |
pCOLD I/adenine deaminase | PMID: 30526847 | Overexpression of the recombinant B. subtilis adenine deaminase in E. coli BL21 (DE3) |
|
| Strain, strain background (B. subtilis) |
B. subtilis | Sigma-Aldrich | ATCC:6633 | |
| Strain, strain background (E. coli) |
E. coli BL21 (DE3) | Agilent Technologies | 200131 | |
| Chemical compound, drug |
S-[methyl-3H]adenosyl-L-methionine; [3H]SAM | PerkinElmer | NET155V250UC; NET155V001MC |
|
| Software, algorithm |
GraphPad Prism | GraphPad Software | RRID:SCR_002798 | |
| pET28-MHL | Vector | Addgene | 26096 (Genbank accession number: EF456735) |
Expression of SETD3 and its mutants |
| Origin 6.1, Origin 7.0 |
Software | OriginLab | http://www.originlab.com/ | For ITC curve fitting and calculation of Kd values |
| HKL2000 | Software | PMID: 27754618 | http://www.hkl-xray.com/ | Processing the crystal structure data |
| Phaser | Software | PMID: 19461840 | http://www.phaser.cimr.cam.ac.uk/ | Molecular replacement |
| Phenix | Software | PMID: 20124702 | http://www.phenix-online.org/ | Structure refinement |
| Coot | Software | PMID: 15572765 | http://www2.mrc-lmb.cam.ac.uk/Personal/pemsley/coot/ | Structure refinement |
| Pymol | Software | DeLano Scientific LLC | http://www.pymol.org/ | Making structure figures |
| Molprobity | Software | PMID: 20057044 | http://molprobity.biochem.duke.edu/ | Structure validation |