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. Author manuscript; available in PMC: 2019 Mar 6.
Published in final edited form as: Nature. 2018 Sep 5;561(7723):331–337. doi: 10.1038/s41586-018-0499-y

ED-Figure 5 │. Flow-cytometry quantification of nodes downstream of CD6 signaling endodomains and high-throughput analysis of super-resolution imaging using deconvolution microscopy (DM).

ED-Figure 5 │

Quantification of the flow cytometric data for (A) LFA-1 open configuration, (B) pZap70 and (C) Talin before (solid bars) and after (dotted bars) TWM of 1×105 T-cells. *** p<0.001. (D) Characterization of migrant T cells cellular features using collective quantification of Actin MFI, focal adhesions at HS/ALCAM interface, area of spreading, and podosynapse formation by high throughput microscopy in 3 donors. n=200–800 cells. (E) Box plot summary representing single cell data distributions of all replicates between all three donors expressing HS vs. NT controls.