Fig. 1.

2D-DIGE-based differential proteome analysis of platelets from obese and lean individuals: up-regulation of fibrinogen features in obese patients.

(A-i) Representative image of the analysis in grey scale. The figure shows the location on the 2D gels of those spots that are differentially regulated when comparing obese patients and lean matched-controls. Protein identifications are shown by the identification numbers in Supplementary Tables 3 and 4. Spots corresponding to fibrinogen are indicated with a black arrow. (A-ii) Enlarged images of representative fibrinogen spots found to be up-regulated in obese patients by proteomics. (B) Representative 2D-Western blot images of fibrinogen on pools of samples from obese-patients’ platelets and lean matched-controls (n = 22 per group). GAPDH was used as loading control (1D-western blot). Western blot experiments were run in triplicate. OB: severe obese patients; C: lean matched-controls; IB: immunoblot.