FIG 3.

(A) Comparison of linear plasmids carrying different selection markers with and without ORI410: transformation efficiency, proportion of different types of transformants, and log₂ value of the ratio of average number of transient or autonomous transformants with ORI to that without ORI (ORI410/No ORI). CaURA3, CaHIS1, CdARG4, and CmLEU2 plasmids were transformed in C. albicans BWP17, SN76, SN76, and SN152, respectively. Different markers are represented by different colors, and different types of transformants are represented by various shades of a color (lightest shade representing transients, intermediate shade representing ARS-transformants, and darkest shade representing integrants). The transformation efficiency is an average from three independent experiments. The size of circles showing proportion of transformant types is an approximation of transformation efficiency. (B) Mitotic stability (%) of integrants and ARS-transformants and plasmid loss rate/generation for ARS-transformants obtained with different linear plasmids with and without ORI410. The data represent the average from three independent colonies of each type except for ARS-transformants with CdARG4 and CmLEU2 plasmids, where they represent the average from six independent colonies. Int, integrants; ARS, ARS-transformants.