Table I.

The streamlined panel of agonists used for lumi-aggregometry in diagnosing platelet function defects in 175 women with HMB. ATP secretion from dense granules was measured with ADP 30 µM, adrenaline 10 µM, arachidonic acid 1 mM, PAR1 – specific peptide 100 µM and collagen 3 µg/ml.

Agonist	Concentration	Comments/further guidance
ADP	10 µM	Maximal, sustained aggregation expected at this concentration. If reversible aggregation seen use 30 µM.
Adrenaline	10 µM	Biphasic aggregation expected. If aggregation absent or reduced use 30 µM.
Arachidonic acid	1 mM	Maximal, sustained aggregation expected. If aggregation reduced or reversible use U46619 3 µM.
PAR-1 receptor specific peptide (SFLLRN)	100 µM	Maximal, sustained aggregation expected. If aggregation reduced use PAR-4 receptor specific peptide (AYPGKF; 500 µM).
Collagen	1 µg/ml	If aggregation reduced use 3 µg/ml. If this concentration shows reduced aggregation, use collagen related peptide (CRP) 3 µg/ml.
Ristocetin	1.5 mg/ml	Maximal, sustained agglutination (often biphasic) expected. If reduced consider vWF profile and GPIb levels by flow cytometry.