Figure 2. Effects of baicalin on oxidative stress in bovine MEC after two treatment duration.

The effects of baicalin treatment were evaluated under different concentrations (0–200 µg/mL) during 90 min (A) and 24 h (B). The Reactive Oxygen Species (ROS) production was determined by a fluorimetric assay using five μM of 2′,7′-Dichlorofluorescin diacetate (DCFDA) added to the different media. After incubation, the hydroxyl radicals (ROS) produced by the cells were estimated by quantifying oxidation fluorescent product 2′,7′-Dichlorofluorescein (DCF). The oxidative stress is expressed as light intensity in percentage of the MFI control (0 µg/mL baicalin). *p < 0.05 versus control.