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. Author manuscript; available in PMC: 2020 Mar 1.
Published in final edited form as: Gastroenterology. 2018 Dec 3;156(4):1052–1065. doi: 10.1053/j.gastro.2018.11.061

Figure 2. Effects of fasting and refeeding on intestinal expression of NPC1L1 in WT and SHP-knockout mice.

Figure 2.

(A-C) WT and SHP-knockout (KO) mice were fasted overnight and refed normal chow for 6 h, and mRNA (A) and protein (B) levels in the pooled jejunum and ileum were measured. (C) NPC1L1 protein was detected by immunohistochemistry in sections of the jejunum. (D-E) WT and SHP-knockout mice were fasted overnight and refed normal chow for the indicated times, and pre-mRNA and protein levels of NPC1L1 in the pooled jejunum and ileum were determined by RT-qPCR and IB, respectively for WT (D) and SHP-knockout (E) mice. (A, B, D, E) Statistical significance was determined by (A, B) two-way or (D, E) one-way ANOVA with the FDR post-test, (SEM, n=3 to 5, *P <0.05, **P <0.01, NS, statistically not significant).