Fig. 1.

The AR protein, transactivity and AR downstream DDR gene expression levels are elevated after IR. (a) After IR the AR protein level increased in a time dependent-manner (upper panels) in C4-2 and CWR22Rv-1 cells. Treating cells with ASC-J9® (J9) and IR can inhibit AR increase (lower panels). Enz treatment does not show the similar effect (middle panels). (b) The qPCR results show AR RNA levels increased in C4–2 cells after IR. (c) Luciferase activity reporter assays demonstrate that IR increases AR transactivity in C4-2 cells. (d) The qPCR results of several AR target genes and downstream DDR genes expressions are elevated in C4–2 cells after IR.For b-d, data are presented as Mean ± SEM, *P < 0·05, **P < 0·01.