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. 2019 Feb 15;24(4):707. doi: 10.3390/molecules24040707

Figure 8.

Figure 8

Short-term and direct effect of xanthone OX6 on P-glycoprotein (P-gp) activity, evaluated ex vivo. P-gp-mediated Rhodamine 123 (Rho 123) efflux was measured using 10 cm everted sacs from distal ileum. The sacs were filled with 300.0 μM Rho 123 (serosal side). The dye secreted into the outside compartment (mucosal side) was assessed every 5 min for 45 min, in the presence or absence of 20.0 μM OX6 and/or 10.0 μM zosuquidar (Zos). Data are expressed as means ± SEM of five to eight rats per group. The excreted amounts of Rho 123 into the mucosal side were evaluated by spectrofluorometry, using a Rho 123 calibration curve, and expressed as pmol of Rho 123 transported per mg of tissue. Statistical comparisons were made using two-way ANOVA followed by the Tukey’s multiple comparisons post hoc test (* p <0.05; ** p <0.01; *** p < 0.001; **** p < 0.0001 vs. control; # p < 0.05; ### p < 0.001; #### p < 0.0001 OX6 vs. OX6 + Zos).