Figure 2.
ERα recruitment to chromatin at distinct ER binding sites in response to E2, zearalenone and apigenin. MCF-7 cells were treated with solvent (white) as a negative control, 10−9 M E2 (blue) as a positive control, 10−8 M zearalenone (red) or 10−5 M apigenin (green) for 1 h. The recruitment of ERα to the GREB1 promoter (A), CUE domain containing 1 (CUEDC1) enhancer (B) and the two enhancers of x-box protein 1 (XBP1) (C) was assessed by chromatin immunoprecipitation followed by real-time PCR. For each binding site tested, the DNA sequence is indicated. The results are expressed in fold recruitment compared to control and are the means of four independent experiments. * indicates a p-value < 0.05 and ** indicates a p-value < 0.01 by one-way ANOVA followed by Dunnett’s post hoc test for comparison of the control treatment with the other treatments.