Bone mineral density (BMD) of the femur was obtained from sham and OVX mice fed a control diet, 20% high-amylose corn starch (HAS)-supplemented diet (OVX + HAS), or 20% acid-hydrolysed high-amylose corn starch (AH-HAS)-supplemented diet (OVX + AH-HAS) for 42 days. BMD of the (A) whole femur (B) proximal femur, (C) middle femur and (D) distal femur were analysed by DXA. BMD of the (E) Cortical and (F) trabecular bone, and (G) bone volume per tissue volume (BV/TV) on distal femur were analysed by uCT. Values are the mean ± SEM (n = 8). Significant differences in BMDs were determined by analysis of covariance (ANCOVA) and the Bonferroni significant difference test. Differences were considered significant if p < 0.05. Body weight was used as a covariate in the analysis of BMDs to adjust for a possible confounding effect. a, b, c Mean values with unlike letters are significantly different.