Figure 3.

Effect of PRFR on survival protein expression in HepG2 cells. The cells were incubated with or without PRFR (0–25 µg/mL) for 24 h (a) and 48 h (b). The expression of survival proteins regulating the cell cycle was detected by western blot analysis. The band intensity has been shown as a relative ratio of the interested protein to β-actin. Data from a typical experiment are depicted here and similar results were obtained in three independent experiments.