Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Antibody | Mouse monoclonal Anti-c-Myc | Millipore Sigma | Cat#M4439; Clone#9E10 | (1:5000) |
| Antibody | Peroxidase Affinipuregoat anti-mouse IgG | Jackson Immunoresearch | Cat#115-035-146 | (1:10000) |
| Chemical compound, drug | n-dodecyl-β-d-maltopyranoside(DDM), Solgrade | Anatrace | Cat#D310S | |
| Chemical compound, drug |
Water for molecular biology | Millipore | H20MB1006 | |
| Chemical compound, drug | Calcium nitratetetrahydrate | Millipore Sigma | Cat#C4955 | |
| Chemical compound, drug | Sodium chloride | Millipore Sigma | Cat#71380 | |
| Chemical compound, drug | Magnesium chloride | Fluka | Cat#63065 | |
| Chemicalcompound, drug | HEPES | Millipore Sigma | Cat#H3375 | |
| Chemicalcompound, drug | Ethyleneglycol-bis(2-aminoethylether)-N,N,N′,N′-tetraacetic acid | Millipore Sigma | Cat#03777 | |
| Chemicalcompound, drug | Ethylenediamin etetraacetic acid |
Millipore Sigma | Cat#E6758 | |
| Chemical compound, drug | cOmplete, EDTA-freeProtease Inhibitor Cocktail | Roche | Cat#5056489001 | |
| Chemical compound, drug | Biotin | Millipore Sigma | Cat#B4501 | |
| Chemical compound, drug | D-desthiobiotin | Millipore Sigma | Cat#D1411 | |
| Chemical compound, drug | Glycerol | Millipore Sigma | Cat#G5516 | |
| Chemical compound, drug |
1-palmitoyl-2-oleoyl-glycero-3-phosphocholine | Avanti Polar Lipids, Inc | Cat#850457C | |
| Chemical compound, drug | 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-(1'-rac-glycerol) | Avanti Polar Lipids, Inc | Cat#840457C | |
| Chemical compound, drug | Diethyl ether | Millipore Sigma | Cat#296082 | |
| Chemicalcompound, drug | E. coli polar lipid extract | Avanti Polar Lipids, Inc | Cat#100600C | |
| Chemicalcompound, drug | Egg PC, 95% | Avanti Polar Lipids, Inc | Cat#131601C | |
| Chemical compound, drug | 18:1-06:0 NBD-PE | Avanti Polar Lipids, Inc | Cat#810155C | |
| Chemicalcompound, drug | Potassium chloride | Millipore Sigma | Cat#746436 | |
| Chemicalcompound, drug | CHAPS | Millipore Sigma | Cat#C3023 | |
| Chemical compound, drug | Sodium dithionite | Millipore Sigma | Cat#157953 | |
| Chemical compound, drug | Yeast nitrogen base without amino acids | Millipore Sigma | Cat#Y0626 | |
| Chemicalcompound, drug | Yeast SyntheticDrop-out MediumSupplements without uracil | Millipore Sigma | Cat#Y1501 | |
| Chemicalcompound, drug | D-Glucose | Applichem | Cat#A1422 | |
| Chemical compound, drug | Galactose | Millipore Sigma | Cat#G0625 | |
| Chemical compound, drug | Lithium acetate | Millipore Sigma | Cat#L4158 | |
| Chemical compound, drug |
PEG3350 | Millipore Sigma | Cat#88276 | |
| Peptide,recombinant protein | DNAse I | AppliChem | A3778-0500 | |
| Peptide, recombinant protein |
HRV 3C protease | Raimund Dutzler laboratory | ||
| Commercial assay or kit |
Pierce StreptavidinPlus UltraLink Resin | Thermo Fisher Scientific | Cat#53117 | |
| Commercial assay or kit |
Bio-Beads SM-2 Adsorbents |
Bio-Rad | Cat# 1523920 | |
| Commercialassay or kit | Amicon Ultra-4–100 KDa cutoff | EMD Millipore | Cat#UFC8100 | |
| Commercialassay or kit | 0.22 μm Ultrafree-MC Centrifugal Filter |
EMD Millipore | Cat#UFC30GV | |
| Commercialassay or kit | Strep-Tactin Superflow high capacity | IBA Lifesciences | Cat#2-1208-010 | |
| Commercial assay or kit | Superdex 200 10/300 GL | GE Healthcare | Cat# 17-5175-01 | |
| Commercial assay or kit | Superose 6 10/300 GL | GE Healthcare | Cat#17-5172-01 | |
| Strain, strainbackground(S. cerevisiae) | FGY217 | David Drew laboratory | ||
| Recombinant DNA reagent | nhTMEM16 open reading frame | GenScript | PubMed accession number XM_003045982 | |
| RecombinantDNA reagent | Yeast expressionvector withN-terminal streptavidinbinding peptide,Myc tag and 3C protease cleavage site | Raimund Dutzler laboratory | ||
| Recombinant DNA reagent | Membrane scaffoldprotein (MSP) 2N2 | Stephen Sligar laboratory | Addgene:Cat#29520 | |
| Software, algorithm | Online WEBMAXC calculator | Bers et al., 2010 | http://maxchelator.stanford.edu/webmaxc/webmaxcS.htm | |
| Software, algorithm | Focus 1.1.0 | Biyani et al. (2017) | https://focus.c-cina.unibas.ch/about.php | |
| Software,algorithm | MotionCorr2 1.1.0 | Zheng et al. (2017) | http://msg.ucsf.edu/em/software/motioncor2.html | |
| Software, algorithm | CTFFIND 4.1 | Rohou and Grigorieff, 2015 | http://grigoriefflab.janelia.org/ctf | |
| Software, algorithm | Relion v 2.1 and 3.0 | Kimanius et al., 2016 | https://www2.mrc-lmb.cam.ac.uk/relion/ | |
| Software, algorithm | Phenix 1.14 | Adams et al., 2010 | http://phenix-online.org/ | |
| Software, algorithm | Coot 0.8.9.1 | Emsley and Cowtan, 2004 | https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/ | |
| Software, algorithm | Pymol 2.0 | Schrodinger LLC | https://pymol.org/2/ | |
| Software, algorithm | Chimera 1.12 | Pettersen et al., 2004 | https://www.cgl.ucsf.edu/chimera/ | |
| Software, algorithm | ChimeraX 0.6 | Goddard et al., 2018 | https://www.rbvi.ucsf.edu/chimerax/ | |
| Other | Whatman Nuclepore Track-Etched Membranes diam. 19 mm, pore size 0.4 μm, polycarbonate |
Millipore Sigma | Cat#WHA800282 | |
| Other | HPL6 | Maximator | ||
| Other | Fluoromax 4 | Horiba | ||
| Other | 300 mesh Au 1.2/1.3 cryo-EM grids | Quantifoil | Cat#N1-C14nAu30-01 |