Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Mar 12;10(2):e01991-18. doi: 10.1128/mBio.01991-18

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2019 Edwards et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

PMC Copyright notice

FIG 2 — RstA inhibits toxin gene expression. (A) A schematic of the promoter regions of tcdR, tcdA, and tcdB denoting the relative locations of the transcriptional start sites experimentally demonstrated (12, 32 –34) and the open reading frames of all three genes (not drawn to scale). Pale red boxes approximate CodY- and CcpA-binding sites within the toxin gene promoters (8, 9, 36). The yellow boxes indicate the locations and sizes of the promoter fragments constructed for the phoZ reporter fusions in panels B to D. Alkaline phosphatase (AP) activity of the PtcdR::phoZ reporter fusions of various lengths (B) (promoterless phoZ [MC448], PtcdR_σA [MC1285/MC1286], PtcdR_σD [MC1145/MC1146], PtcdR_σTcdR(P2) [MC1147/MC1148], and PtcdR_σTcdR(P1) [MC1149/MC1150]) and the PtcdA::phoZ (C) (−511 bp to −1 bp upstream of transcriptional start; MC1249/MC1250) or PtcdB::phoZ (D) (−531 bp to −31 bp upstream of transcriptional start [MC1251/MC1252]) reporter fusions in strain 630Δerm and the rstA::erm mutant (MC391) grown in TY medium (pH 7.4) at H₂₄. The means and standard errors of the means for four biological replicates are shown. *, P < 0.05, using Student’s t test compared to the activity observed in the 630Δerm parent strain for each promoter construct.