Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Antibody | Anti-Connexin-43 (rabbit, polyclonal) |
Cell Signalling | 3512, RRID:AB_2294590 |
WB (1:850) IHC/IF (1:100) |
| Antibody | Anti-β-actin HRP-conjugated (mouse, monoclonal) |
Sigma-Aldrich | A2228, RRID:AB_476697 |
(1:5000) |
| Antibody | Anti-rabbit HRP-conjugated (goat, polyclonal) |
Dako | P0448, RRID:AB_2617138 |
(1:5000) |
| Antibody | Anti-rabbit biotinylated (goat, polyclonal) |
Dako | E0432, RRID:AB_2313609 |
(1:200) |
| Antibody | Anti-rabbit IgG FITC-conjugated (goat, polyclonal) |
Sigma-Aldrich | F0382, RRID:AB_259384 |
(1:200) |
| Cell line (Homo sapiens) |
HEK293-pmeLUC | doi:10.1371/journal.pone.0002599 | ||
| Chemical compound |
ARL 67156 | Sigma-Aldrich | 67156 | |
| Chemical compound |
TRIzol | Roche | 11667165001 | |
| Chemical compound |
Omniscript RT Kit 200 |
Quiagen | 205113 | |
| Chemical compound |
Lipopolysaccharides E.coli O111:B4 |
Sigma-Aldrich | L4130 | |
| Chemical compound |
Pam3CSK4 | Invivogen | tlrlpms | |
| Chemical compound |
18-alpha- glycyrrhetinic acid |
Sigma-Aldrich | G8503 | |
| Chemical compound |
Gap27 | Tocris-Bioscience | 1476 | |
| Chemical compound |
Probenecid | Sigma-Aldrich | P8761 | |
| Chemical compound |
Carbenoxolone | Sigma-Aldrich | C4790 | |
| Commercial assay |
ATP Reagent SL | BioThema | 144–041 | |
| Commercial assay |
Resazurin assay | Sigma-Aldrich | 199303 | |
| Commercial kit |
V-PLEX Mouse Cytokine 19-Plex kit |
Meso Scale Discovery |
K15255D-1 | |
| Commercial kit (ELISA) |
TNF-alpha | Invitrogen | 88-7324-22 | |
| Commercial kit (ELISA) |
IL-6 | Invitrogen | 88-7064-22 | |
| Commercial kit (ELISA) |
IL-10 | Invitrogen | 88-7105-22 | |
| Commercial kit (ELISA) |
IL-1 beta | Invitrogen | 88-7013-86 | |
| Commercial assay |
Phagocytic assay | Cayman Chemical | 500290 | |
| Other | Brewer thioglycollate | Sigma-Aldrich | B2551 | |
| Other | DMEM/F12 GlutaMAX | ThermoFisher Scientific, Gibco |
10565018 | |
| Other | CTAD tubes | BD Vacutainer | 367599 | |
| Other | Peroxidase labelled streptavidin |
Seracare | 71-00-38 | (1:200) |
| Other | DAPI stain | Sigma-Aldrich | D9542 | (1:1000) |
| Other | Ficoll | GE Healthcare | 17-5442-02 | |
| Other | Myeloperoxidase assay |
doi:10.1097/TP.0000000000000101 | ||
| Strain, strain background (Mus musculus) |
Wild-type mice | Harlan, Netherlands | C57BL/6J | |
| Strain, strain background (Mus musculus) |
MyD88/TRIF double-knockout mice |
Mucosal Immunology, University of Bern |
B6.129-MyD88tm1Aki x B6-Ticam1lps2 |
|
| Strain, strain background (Mus musculus) |
Caspase-1/11 double-knockout mice |
Mucosal Immunology, University of Bern |
B6N.129S2-Casp1tm1Flv | |
| Strain, strain background (Mus musculus) |
MAC-CX43 knockout mice |
Visceral Surgery, University of Bern |
B6.129P2-Lyz2tm1(cre)Ifo/J x B6.129S7-Gja1tm1Dlg/J |
Cell-specific conditional MAC-CX43 KO mouse model developed in our laboratory |
| Strain, strain background (Mus musculus) |
Lyz2-cre mice | Jackson Laboratory | B6.129P2-Lyz2tm1(cre)Ifo/J | |
| Strain, strain background (Mus musculus) |
Gja1 floxed mice | Jackson Laboratory | B6.129S7-Gja1tm1Dlg/J |