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. 2019 Feb 26;10(1):166–179. doi: 10.1080/21505594.2019.1582975

Figure 2.

Figure 2.

Confirmation of α-toxoid protein expression by pPG-α/L. casei 393. (a) The cell lysates were analyzed by western blotting with an anti–α-toxoid monoclonal antibody, and the results showed that pPG-α/L. casei 393, but not pPG/L. casei 393 and L. casei 393 expressed this toxoid. (b) Determination of the cell surface expression of the α-toxoid by laser confocal microscopy. The results revealed that there was clear-cut green fluorescence on the cell surface of pPG-α/L. casei 393, but not on pPG/L. casei 393. (c) The cell surface expression of the α-toxoid protein as analyzed by flow cytometry.