Figure 2.

Full-Aptamer-Split-DNAzyme (FaptaSyme) sensor design and performance. A) Design strategy: only the DZ sequence is split in half; each segment is linked to a full aptamer sequence. FaptaSyme-a and FaptaSyme-b strands bind α-syn by their aptameric portions and form a DZ catalytic core followed by fluorescent signaling. In both cases, DZ and aptameric portions were linked by oligoethylene glycol linkers (dashed lines). B) Selectivity of α-syn recognition by the FaptaSyme sensor. All samples contained 200 nM F_sub, 2 nM FaptaSyme-a, 50 nM FaptaSyme-b, and either no protein target (Noise) or 100 nM oligomeric or monomeric α-syn, or 26 μM of thrombin (Negative control). The concentrations of FaptaSyme were optimized, as detailed in Figure S2.