REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Rabbit anti-Brn3a	Millipore	Cat# AB5945; RRID: AB_92154
Chicken anti-NF200	Abcam	Cat# ab4680; RRID: AB_304560
Rabbit anti-HA	Cell Signaling Technology	Cat# 3724; RRID: AB_1549585
Mouse anti-HA	Sigma-Aldrich	Cat# A2095; RRID: AB_257974
Mouse anti-Beta-Actin	Sigma-Aldrich	Cat# A5316; RRID: AB_476743
Rat anti-MBP	Abcam	Cat# ab7349; RRID: AB_305869
Phalloidin-Tetramethylrhodamine B isothiocyanate (TRITC)	Sigma-Aldrich	Cat: P1951; RRID: AB_231514
Rabbit anti-Protein Gene Protein 9.5 (PGP 9.5)	Zytomed	Cat# 516-3340
Amersham ECL Mouse IgG, HRP-linked whole antibody (from sheep)	GE Healthcare Life Sciences	Cat# NA931V; RRID: AB_772210
Amersham ECL Rabbit IgG, HRP-linked whole antibody (from donkey)	GE Healthcare Life Sciences	Cat# NA934V; RRID: AB_772206
Donkey anti-rabbit IgG Alexa 488	Thermo Fisher Scientific	Cat# A-21206; RRID: AB_2535792
Donkey anti-rabbit IgG Cy3	Jackson ImmunoResearch Labs	Cat# 711-166-152; RRID: AB_2313568
Donkey anti-mouse IgG Alexa 488	Thermo Fisher Scientific	Cat# A-21202; RRID: AB_141607
Goat anti-chicken Alexa 488	Thermo Fisher Scientific	Cat# A-11039; RRID: AB_2534096
Donkey anti-rabbit IgG Alexa 546	Thermo Fisher Scientific	Cat# A10040; RRID: AB_2534016
Goat anti-rabbit IgG Alexa 488	Thermo Fisher Scientific	Cat# A-11008; RRID: AB_143165
Guinea pig anti-CASPR	Gift from Bhat MA	N/A
Mouse anti-S100	Sigma-Aldrich	Cat#S2532; RRID: AB_477499
Goat anti-Rat CF405M	Sigma-Aldrich	Cat#SAB4600463
Biotinylated Goat anti-rabbit	Vector Biolabs	Cat# BA-1000; RRID: AB_2313606
Alexa Fluor 488 streptavidin	Thermo Fisher Scientific	Cat#S11223; RRID: AB_2336881
Bacterial and Virus Strains
Lentivirus CAMKII-EGFP	Signagen	Cat#: SL100304
Chemicals, Peptides, and Recombinant Proteins
BDNF recombinant human	Thermo Fisher Scientific	Cat#10908010
NT3 recombinant human	Peprotech	Cat#450-03
β-NGF recombinant human	Peprotech	Cat#450-01
GDNF recombinant human	Peprotech	Cat#450-10
Cultrex Mouse Laminin I, Pathclear	R&D Systems	Cat#3401-010-02
CHIR99021	Sigma-Aldrich	Cat#SML1046
SU-5402	Sigma-Aldrich	Cat#SML044
DAPT	Sigma-Aldrich	Cat#D5942
LDN-193189	Sigma-Aldrich	Cat#SML0559
SB431542	Sigma-Aldrich	Cat#616461
PF-05089771	Sigma-Aldrich	Cat#PZ0311
BIIB074	Axon Medchem	Cat#2548
Naloxone	Sigma-Aldrich	Cat#N7758
Critical Commercial Assays
Pierce BCA Protein Assay Kit	Thermo Fisher Scientific	Cat#: 23227
Deposited Data
Human iPSC (AD2, AD3, NHDF, AH017) and iPSC-nociceptor RNA-seq data	Baskozos et al., 2019	GEO: GSE107181
Human DRG L2 RNA-seq data	Ray et al., 2018	dbGAP: phs001158.v1.p1
Single cell RNA-seq human iPSC and iPSC-nociceptor data	Schwartzentruber et al., 2018	ENA: ERP020576
Human whole blood, skin, skeletal muscle, fibroblasts, and tibial nerve raw counts	GTEx V7	N/A
Experimental Models: Cell Lines
Human iPSC line_ HC1	StemBANCC Consortium	AD2-1
Human iPSC line_ HC2	StemBANCC Consortium	NHDF-1
Human iPSC line_HC3	StemBANCC Consortium	AH017-7
Human iPSC line_CIP1.1	StemBANCC Consortium	811-05-01
Human iPSC line_CIP1.2	StemBANCC Consortium	811-05-03
Human iPSC line_CIP2	StemBANCC Consortium	246-03-01
Oligonucleotides
YWHAZ F 5′-CCTGCATGAAGTCTGTAACTGAG-3′	IDT	N/A
YWHAZ R 5′-GACCTACGGGCTCCTACAACA-3′	IDT	N/A
SCN9A F 5′-GGCATAGGCGAGCACATGAA-3′	IDT	N/A
SCN9A R 5′-AACAAGGAGCCACGAATGCT-3′	IDT	N/A
Recombinant DNA
pSpCas9(BB)-2A-Puro (PX459) V2.0	Addgene (Depositor:Feng Zhang)	#62988
Software and Algorithms
ImageJ/Fiji	NIH	https://imagej.nih.gov/ij/index.html, https://fiji.sc/
Clampfit 10	Molecular Devices	http://mdc.custhelp.com/app/answers/detail/a_id/18779/?/axon%E2%84%A2-pclamp%E2%84%A2-10-electrophysiology-data-acquisition-%26-analysis-software
Prism 7.0	GraphPad software	https://www.graphpad.com/
FMRIB’s Software Library	Smith et al., 2004	https://fsl.fmrib.ox.ac.uk/fsl/fslwiki
QTRAC software	Institute of Neurology, London, UK	N/A
STAR version 2.5.2b	Dobin et al., 2013	https://github.com/alexdobin/STAR
HTSeq version 0.11.0	Anders et al., 2015	https://github.com/simon-anders/htseq
DESeq2 version 1.18.1	Love et al., 2014	https://www.bioconductor.org/packages/release/bioc/html/DESeq2.html
GTEx portal version 7	Carithers and Moore, 2015	https://gtexportal.org/home/
R version 3.4.4	R Development Core Team, 2018	https://cran.r-project.org/
Ggplot2 version 2.2.1	Wickham, 2009	https://cran.r-project.org/web/packages/ggplot2/index.html
IN Cell Developer Toolbox analysis software build 1.9.2	GE	IN Cell Developer Toolbox analysis software build 1.9.2