FIG 4.

Crossroads is the only subcluster L2 phage targeted by Sbash gp30/gp31 defense. (A) Genome maps of subcluster L2 phages, Crossroads, Kahlid, LilDestine, Wilder, Gardann, MkaliMitinis3, Faith1, BigCheese, and Rumpelstiltskin (top to bottom). Genomes are represented as described for Fig. 1. (B) Expanded view of the right genome ends of phages BigCheese, Crossroads, Wilder, and Rumpelstiltskin. Crossroads and Wilder were very similar in this region, whereas BigCheese and Rumpelstiltskin have 3-kbp to 5-kbp deletions. (C) Plating efficiencies of subcluster L2 phages on mc²155 and a Sbash lysogen. Ten-fold serial dilutions of each phage were plated as indicated.