TABLE 1.
Defense escape mutants of phage Crossroads
| Mutant name | Defender | Length (bp) |
Mutation | Gene (substitution) |
|---|---|---|---|---|
| Crossroads-wt | 76,129 | |||
| Crossroads-par | 71,124 | Δ38089–43093 | Δ49–58 | |
| phgg344 | pGG05 | 71,124 | G68548T | gp132 R185S |
| phgg348 | pGG05 | 71,025 | 74303Δ99 bp | 141–142 intergenic |
| phgg350 | pGG05 | 71,124 | A73608T | gp141 V214E |
| phgg354 | pGG05 | 71,133 | 68435↑9 bp (dup) | gp132 222↑HDK |
| phgg356 | pGG05 | 71,125 | 68575↑1 bp | gp132 175f/s |
| phgg359 | pGG05 | 71,118 | Δ73760–73765 (Δ6 bp) | gp141 ΔE161, ΔT162 |
| phgg364 | pGG05 | 71,124 | Δ73760–73765 (Δ6 bp) | gp141 ΔE161, ΔT162 |
| phgg398 | Sbash | 71,125 | 68395↑1 bp | gp132 236f/s |
| phgg399 | Sbash | 71,124 | G68231T | gp141 A338D |
| phgg400 | Sbash | 71,124 | G68548T | gp132 R185S |
| phgg403 | Sbash | 71,124 | G73236T | gp141 A338D |
| phgg407 | Sbash | 71,124 | G73275T | gp141 A325E |
| phgg408 | Sbash | 71,133 | 68435↑9 bp (dup) | gp132 222↑HDK |
| phgg409 | Sbash | 71,124 | C73681A | gp141 V190F |
| phgg411 | Sbash | 71,124 | G73317T | gp141 A311E |
| phgg413 | Sbash | 71,133 | 74335↑9 bp (dup) | 141–142 intergenic |
| phgg417 | Sbash | 71,124 | T74303C | 141–142 intergenic |
| phgg419 | Sbash | 71,150 | 74312↑26 bp (dup) | 141–142 intergenic |
| 4_1A | Sbash | 71,124 | G73437A | gp141 S271F |
| 3_4A | Sbash | 71,118 | Δ73760–73765 (Δ6 bp) | gp141 ΔE161, ΔT162 |
| 3_3A | Sbash | 71,124 | A73009G | gp141 *413Q |
| 1_4A | Sbash | 71,118 | Δ73760–73765 (Δ6 bp) | gp141 ΔE161, ΔT162 |
| 1_3A | Sbash | 71,118 | Δ73760–73765 (Δ6 bp) | gp141 ΔE161, ΔT162 |
| 1_2A | Sbash | 71,124 | G73101T | gp141 A383E |