TABLE 2.
Fluconazole-induced MTL-homozygous strains used in mating experiments
| Strain | Parent | Relevant genotypea | Fluconazole MIC (µg/ml) |
|---|---|---|---|
| SCERG11R32hom1A | SCERG11R32A | ERG11G464S/ERG11G464S; MTLα/α; GLN3-1/GLN3-1 | 8 |
| SCERG11R32hom1B | SCERG11R32B | ERG11G464S/ERG11G464S; MTLa/a; GLN3-1/GLN3-2 | 8 |
| SCMRR1R32hom1A | SCMRR1R32A | MRR1P683S/MRR1P683S; MTLα/α; GLN3-1/GLN3-1; CAP1-1/CAP1-2 | 16 |
| SCMRR1R32hom2A | SCMRR1R32A | MRR1P683S/MRR1P683S; MTLa/a; GLN3-2/GLN3-2; CAP1-1/CAP1-2 | 16 |
| SCTAC1R32hom2A | SCTAC1R32A | TAC1G980E/TAC1G980E; MTLα/α; GLN3-1/GLN3-2 | 8 |
| SCTAC1R32hom2B | SCTAC1R32B | TAC1G980E/TAC1G980E; MTLa/a; GLN3-1/GLN3-2 | 8 |
| SCUPC2R12hom1A | SCUPC2R12A | UPC2G648D/UPC2-2; MTLa/a; GLN3-2/GLN3-2 | 2 |
| SCUPC2R12hom1B | SCUPC2R12B | UPC2G648D/UPC2-2; MTLα/α; GLN3-1/GLN3-1 | 2 |
a
The two GLN3 alleles on the right arm of Chr5 in strain SC5314 were distinguished by a ClaI restriction site polymorphism; the GLN3 allele with the upstream ClaI site at position −1712 (on Chr5B) was designated GLN3-1 (67). The two CAP1 alleles on the left arm of Chr3 in strain SC5314 were distinguished by an EcoRI restriction site polymorphism; the CAP1 allele with the EcoRI site at position +1212 (on Chr3A) was designated CAP1-2 (62).