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. 2019 Mar 4;24(5):896. doi: 10.3390/molecules24050896

Figure 4.

Figure 4

Measurement of AChE activity. (A) After homogenization of colon tissue, AChE activity was measured using an Acetylcholinesterase Assay Kit that could detect as little as 0.01 mU AChE in a 100 μL assay volume (0.1 mU/mL). Five to six mice per group were used in preparation of tissue lysate and enzyme activity was assayed in duplicate in each test. (B) The expression of Bax, Bcl-2 and CD34 protein representing apoptotic phenomena were measured by Western blot analysis using horseradish peroxidase (HRP)-labeled anti-rabbit IgG antibody. Five-to-six mice per group were used in preparation of total lysate and specific protein level was assayed in duplicate in each test. Data are reported as the mean ± SD. *, p < 0.05 compared to the untreated group. #, p < 0.05 compared to the Lop + Vehicle treated group. No, Untreated group; Lop, Loperamide; SpiA, Spicatoside A; AChE, Acetylcholinesterase, HRP; Horseradish peroxidase.