Correction to Figure 2C: In the original Figure 2C, the data showing the tubulin loading controls in the lungs of wild-type and ESKO animals are the same as those in Figure S2F showing tubulin loading controls in the thymus, but in the opposite orientation. In looking through the raw data, the authors realized this was a mistake that happened during figure preparation. Four different western membranes (SIRT1 and tubulin blotting for lungs and thymus) were exposed in a single film where the lung anti-SIRT1 membrane was placed in an inverted direction before exposure. In aiming to ensure that the appropriate lanes were being compared between wild-type and ESKO animals, the authors accidentally used the tubulin data for the thymus instead of the lung and aligned the data accordingly. This was the reason for the difference in orientation but also why the thymus tubulin data seem to have been used twice. The authors have now remade Figure 2C with the correct controls as shown.
Correction to Figure 2D: In the original Figure 2D, the quadriceps sections showing CD31 and laminin staining in young and old animals are the same as the gastrocnemius sections shown in Figure 1D. The correct images for the gastrocnemius and quadriceps sections were provided as part of a single figure during initial submission of this manuscript, and this error occurred during revision as the authors split the images across two figures. The data for these figures were collected in a blinded fashion across multiple biological replicates (eight mice per genotype, with two histological samples from the midsection of each muscle per mouse for both wild-type and ESKO animals). All images were captured at the same time with the same exposure settings. A corrected figure for 2D is included.
The raw data and the replicates for both figures have been uploaded in Mendeley and can be accessed at https://doi.org/10.17632/8rrgmgdtxr.1.
In addition to these two figures, the authors wish to clarify that one of the corresponding authors, Leonard Guarente, is a founder of Elysium Health. In the Declaration of Interests section of the original version of the paper, he was listed as an advisor of the company. The figures and the Declaration of Interests have been corrected online. The authors apologize for any confusion that their errors may have caused.
Figures 2C and 2D. Endothelial SIRT1 Deletion Mimics the Effect of Aging on Capillary Density and Endurance (corrected)
Figures 2C and 2D. Endothelial SIRT1 Deletion Mimics the Effect of Aging on Capillary Density and Endurance (original)